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The operation steps of embedding method vary depending on the specific method, but generally include the following steps: material selection and preparation, mixing of target substance and carrier, polymerization or solidification, subsequent processing, etc. Taking gypsum embedding in dentistry as an example, the first step is to select a suitable casting ring and gypsum binder embedding material, then clean and dry the wax mold, apply the embedding material, cover the casting ring and inject the embedding material, and gently tap the casting ring to remove any bubbles. After the embedded material solidifies, subsequent casting operations can be carried out< In the field of chemical engineering, the operational steps of embedding method may include preparation of enzymes or cells, selection and preparation of carrier materials, mixing, polymerization or solidification of enzymes or cells with carriers, collection and processing of immobilized enzymes or immobilized cells, etc.

Basic principles of embedding method

The basic principle of embedding method is to wrap the target substance in another material through physical or chemical methods. This wrapping process can be achieved through polymerization, ion network formation, precipitation, or changes in solvent, temperature, pH value, and other methods. The buried target substance forms a relatively independent space in the wrapping material, which can prevent its leakage while maintaining its original activity and function. For example, in dentistry, embedding method involves wrapping teeth or tooth models with specific materials such as plaster, phosphate, etc., forming a hard protective layer to fix and protect the teeth; In the field of chemistry and chemical engineering, the embedding method involves embedding enzymes or cells in polymer carriers to form immobilized enzymes or immobilized cells, in order to improve their stability and reusability.

Precautions in the preparation of embedded specimens

When making embedded specimens, the following points should be noted: firstly, ensure that all experimental equipment and reagents are in a clean and sterile state; Secondly, strict adherence to experimental procedures is necessary during the operation process to avoid cross contamination and experimental errors; In addition, attention should be paid to controlling experimental conditions such as temperature, humidity, etc. to ensure the accuracy of experimental results; Finally, experimental data should be recorded in a timely manner during the experiment for subsequent analysis and summary.